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Updated: Jun 5, 2026

2D and 3D Chromosome Painting in Malaria Mosquitoes
Published on: January 6, 2014
Chromosome painting for plant biotechnology
Akio Kato1, Jonathan C Lamb, Patrice S Albert
1Division of Biological Sciences, University of Missouri, Columbia, MO, USA.
Abstract:
Fluorescence in situ hybridization (FISH) is an invaluable tool for chromosome analysis and engineering. The ability to visually localize endogenous genes, transposable elements, transgenes, naturally occurring organellar DNA insertions - essentially any unique sequence larger than 2 kb - greatly facilitates progress. This chapter details the labeling procedures and chromosome preparation techniques used to produce high-quality FISH signals on somatic metaphase and meiotic pachytene spreads.
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