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Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
Published on: March 21, 2018
Electrochemical aptasensor using the tripropylamine oxidation to probe intramolecular displacement between target and
Dong-Yuan Liu1, Yue Zhao, Xi-Wen He
1Research Center for Analytical Sciences, College of Chemistry, Nankai University, 94 Weijin Road, Tianjin 300071, China.
Biosensors & Bioelectronics
|December 25, 2010
Summary
A novel biosensor detects lysozyme by monitoring tripropylamine (TPA) oxidation changes. This method offers a sensitive and direct approach for analyzing protein concentrations in complex samples.
Area of Science:
- Electrochemistry
- Biosensors
- Biochemistry
Background:
- Tripropylamine (TPA) exhibits differential oxidation efficiency on double-stranded (ds)-DNA and single-stranded (ss)-DNA modified electrodes.
- This property can be leveraged for developing sensitive biosensing platforms.
- Lysozyme detection is crucial in various biological and food safety applications.
Purpose of the Study:
- To develop a novel, sensitive biosensor for detecting lysozyme.
- To utilize the differential oxidation of TPA on ds-DNA and ss-DNA for probing molecular interactions.
- To demonstrate the biosensor's efficacy in analyzing real-world samples like egg white.
Main Methods:
- Immobilization of an anti-lysozyme aptamer's complementary strand onto a gold electrode.
- Formation of ds-DNA upon aptamer incubation.
- Lysozyme-induced displacement of the complementary strand, leading to ds-DNA dissociation.
- Detection via TPA oxidation current changes at the modified electrode.
Main Results:
- The biosensor demonstrated high sensitivity for lysozyme detection, with a linear range from 1.0 pM to 1.1 nM.
- Detection of as low as 10 amol (6.0 × 10^6 molecules) of lysozyme was achieved.
- The method showed excellent specificity, stability, and time-saving advantages, avoiding complex sample pre-treatment and DNA labeling.
- Validation with diluted egg white samples yielded high recovery rates (93.3-100%) and good reproducibility (1.4-4.2%).
Conclusions:
- A simple, sensitive, and stable biosensor for lysozyme detection based on TPA oxidation and DNA displacement has been successfully developed.
- The proposed method offers a promising alternative for rapid and efficient analysis of lysozyme in complex matrices.
- This approach avoids cumbersome labeling steps and sample preparation, enhancing its practical applicability.
