Botanical micelle and its application for direct electrochemical biosensor.

Area of Science:

• Biotechnology
• Electrochemistry
• Biosensor Development

Background:

• Enzyme immobilization is crucial for biosensor performance.
• Developing biocompatible and efficient microenvironments for enzymes remains a challenge.
• Horseradish peroxidase (HRP) is a key enzyme for electrochemical biosensing applications.

Purpose of the Study:

• To develop a novel enzyme biosensor using inositol hexakisphosphoric (IP(6)) micelles for horseradish peroxidase (HRP) entrapment.
• To investigate the direct electron transfer between immobilized HRP and the electrode surface.
• To evaluate the performance of the HRP-IP(6) micelle biosensor for hydrogen peroxide (H2O2) detection.

Main Methods:

• Entrapment of HRP within IP(6) micelles.
• Fabrication of an enzyme biosensor utilizing the HRP-IP(6) micelle complex.
• Electrochemical characterization and H2O2 detection using cyclic voltammetry and amperometry.

Main Results:

• Successful entrapment of HRP within IP(6) micelles, creating a biocompatible microenvironment.
• Achieved direct electron transfer between HRP and the electrode surface.
• The HRP-IP(6) biosensor demonstrated a low detection limit of 0.1 μmol L(-1) for H2O2, a rapid response time of 3s, and long-term stability.
• An apparent Michaelis-Menten constant of approximately 0.0016 mmol L(-1) was observed.

Conclusions:

• IP(6) micelles provide an effective and biocompatible matrix for HRP immobilization.
• The developed enzyme biosensor offers high sensitivity, rapid response, and excellent stability for H2O2 detection.
• This approach represents a promising strategy for advanced enzyme biosensor preparation.