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Updated: Jun 5, 2026

Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization
Published on: May 15, 2018
Whole gene amplification and protein separation from a few cells
Bor-Han Chueh1, Cheuk-Wing Li, Huiling Wu
1Department of Chemistry, Stanford University, Stanford, CA 94305, USA.
Abstract:
Despite the growing interest to explore untapped microbial gene and protein diversity, no single platform has been able to acquire both gene and protein information from just a few cells. We present a microfluidic system that simultaneously performs on-chip capillary electrophoresis for protein analysis and whole genome amplification (WGA), and we demonstrate this by doing both for the same cohort of cyanobacterial cells. This technology opens avenues for studying protein profiles of precious environmental microbial samples and simultaneously accessing genomic information based on WGA.
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