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Updated: Jun 5, 2026
Isolation of Human Umbilical Vein Endothelial Cells (HUVEC)
Published on: April 28, 2007
[An improved method of culturing human umbilical vein endothelial cells and its characterization]
Shaofen Lin1, Huanjiao Zhou, Yungang Ding
1State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
Purpose:
To establish a simple and convenient method for the culture of human umbilical vein endothelial cells (HUVECs) and study its characterization in vitro.
Methods:
Human Umbilical cord was isolated and digested by collagenase type I,and then it was cultured in 1.5% geltain coated dish with 10% fetal bovine serum(FBS)，heparin sodium andβ-endothelial cell growth factor(β-ECGF) in human endothelial basal growth medium．HUVECs were identified by anti-human factor Ⅷ related antigen and CD31 immunohistochemical staining．
Results:
We could successfully culture HUVECs by using this method. HUVECs attached the dish in 24 hours and the confluence was seen in 5 days. HUVECs were generally positive for anti-human factor Ⅷ related antigen and CD31．
Conclusion:
It is a fast and effective method to successfully culture purified HUVECs in which collagenase type I was used to digest HUVECs with glass tube connected to T-tube, human endothelial basal growth medium containing 10% FBS,heparin sodium and β-ECGF in 1.5% geltain coated dish.
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