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[Development of a rapid test kit for antibody to HIV by nano immunomagnetic lateral flow method]
Fa-qing Yang1, Tony Lee, Chao-nan Wang
1Newscen Coast bio pharmaceutical Co., Ltd., Tianjin 300457, China.
Objective:
To develop a rapid test kit for antibody to HIV by nano immunomagnetic lateral flow method.
Methods:
A rapid test kit was developed by conjugation of the HIV antigen gp41 and gp36 to 200nm super paramagnetic particles by carbodiimide (EDC) and coating of the HIV antigen gp41 and gp36 to nitrocellulose membrane. Then the kit was evaluated with serials of experiments.
Results:
The kit was qualified with examination of national reference panel of anti-HIV antibody for colloidal gold diagnostic kit. The sensitivity was 100% by tested with 20 HIV antibody positive sera, the specificity was 98.5% by tested with 600 HIV antibody negative sera, respectively. The stability of the kit was over 12 month by storage at room temperature.
Conclusion:
A diagnostic kit for antibody to HIV was developed with the advantages of convenience, rapid test, good stability and point of care.
