Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Circumferential strain recovery after human cardiomyocyte transplantation in minipigs using a novel frequency-based method for myocardial tagging quantification.

Journal of cardiovascular magnetic resonance : official journal of the Society for Cardiovascular Magnetic Resonance·2026
Same author

Cell-type specific allelic dampening of sex-linked genes in sex chromosome aneuploidy.

bioRxiv : the preprint server for biology·2026
Same author

Nanopatterned Cell Sheet Assembly of Biomimetic Cardiac Laminae for Modeling Structure-Function Relationships.

Biomaterials research·2026
Same author

Reliable Identification of Cardiac Maturation Markers Using Robust and Flexible Label-Free Proteomic Quantitation by Spectral Counting on Relatively Abundant Proteins.

Journal of proteome research·2026
Same author

Functional interrogation of contextually correct MYH7 variants using CRaTER-flox gene editing and contractility profiling.

The Journal of clinical investigation·2025
Same author

T Cell Autocrine Hyaluronan Forms Complex Structures in CD4 T Cell Cytoplasm and Plays a Critical Role in Formation of the Immune Synapse.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2025

Related Experiment Video

Updated: Jun 5, 2026

Cardiac Spheroids as in vitro Bioengineered Heart Tissues to Study Human Heart Pathophysiology
10:41

Cardiac Spheroids as in vitro Bioengineered Heart Tissues to Study Human Heart Pathophysiology

Published on: January 23, 2021

Developing vasculature and stroma in engineered human myocardium.

Kareen L Kreutziger1, Veronica Muskheli, Pamela Johnson

  • 1Center for Cardiovascular Biology, Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, Washington, USA.

Tissue Engineering. Part A
|December 29, 2010
PubMed
Summary

Stromal cells, particularly human marrow stromal cells (hMSCs), are crucial for vascularizing engineered heart tissue by modulating the extracellular matrix (ECM). Pro-angiogenic hMSCs, rich in versican, significantly enhance blood vessel formation and host integration.

More Related Videos

Developing 3D Organized Human Cardiac Tissue within a Microfluidic Platform
10:42

Developing 3D Organized Human Cardiac Tissue within a Microfluidic Platform

Published on: June 15, 2021

Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy
11:51

Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy

Published on: March 1, 2016

Related Experiment Videos

Last Updated: Jun 5, 2026

Cardiac Spheroids as in vitro Bioengineered Heart Tissues to Study Human Heart Pathophysiology
10:41

Cardiac Spheroids as in vitro Bioengineered Heart Tissues to Study Human Heart Pathophysiology

Published on: January 23, 2021

Developing 3D Organized Human Cardiac Tissue within a Microfluidic Platform
10:42

Developing 3D Organized Human Cardiac Tissue within a Microfluidic Platform

Published on: June 15, 2021

Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy
11:51

Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy

Published on: March 1, 2016

Area of Science:

  • Biomedical Engineering
  • Cardiovascular Research
  • Stem Cell Biology

Background:

  • Engineered heart tissue requires vascularization for successful engraftment and function.
  • Stromal cells and their extracellular matrix (ECM) production are implicated in supporting vascular development.

Purpose of the Study:

  • To investigate the role of stromal cells in regulating the angiogenic phenotype of engineered heart tissue.
  • To determine if stromal cell-derived ECM modulates endothelial cell organization and vascular network formation.
  • To identify specific stromal cell types and ECM components that promote vascularization.

Main Methods:

  • Development of scaffold-free patches of human myocardium using human embryonic stem cell-derived cardiomyocytes.
  • Co-culture of cardiomyocytes with various stromal cells, including human marrow stromal cells (hMSCs).
  • Assessment of endothelial cell organization and vascular network formation in vitro.
  • Analysis of ECM composition, specifically versican expression.
  • In vivo transplantation of engineered patches onto athymic rat hearts to evaluate vascularization and host integration.

Main Results:

  • Human marrow stromal cells (hMSCs) significantly enhanced endothelial cell organization compared to other stromal cells.
  • Stromal cells produced abundant ECM components, including fibrillar collagen, hyaluronan, and versican.
  • Pro-angiogenic hMSC lines expressed high levels of versican, a proteoglycan known to modulate angiogenesis.
  • Transplanted patches with pro-angiogenic hMSCs showed a ~50-fold increase in human vessel development and formed anastomoses with host circulation.
  • Chimeric vessels containing erythrocytes were observed, indicating successful integration.

Conclusions:

  • Stromal cells play a critical role in supporting the vascularization of engineered human myocardium.
  • Differences in ECM synthesis, particularly versican production, contribute to the varying pro-angiogenic abilities of different stromal cell types.
  • This study provides an in vitro predictive platform for evaluating stromal cell potential in vascular development for tissue engineering applications.