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Related Experiment Video

Updated: Jun 5, 2026

All-optical Mechanobiology Interrogation of Yes-associated Protein in Human Cancer and Normal Cells using a Multi-functional System

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Published on: December 20, 2021

Angiomotin-like proteins associate with and negatively regulate YAP1.

Wenqi Wang¹, Jun Huang, Junjie Chen

¹Department of Experimental Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, Texas 77030, USA.

The Journal of Biological Chemistry

|December 29, 2010

Summary

New research reveals angiomotin-like proteins (AMOTL1 and AMOTL2) regulate the Yes-associated protein YAP1. These interactions control YAP1

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Area of Science:

Cell Biology
Molecular Biology
Genetics

Background:

The Hippo pathway regulates organ size by controlling cell proliferation and apoptosis.
Yes-associated protein YAP1 is a key transcriptional coactivator in this pathway.
YAP1 activity is modulated by its nuclear-cytoplasmic localization, dependent on phosphorylation.

Purpose of the Study:

To identify new YAP1-associated proteins.
To elucidate novel mechanisms regulating YAP1.
To investigate the role of angiomotin-like proteins in YAP1 function.

Main Methods:

Co-immunoprecipitation to identify YAP1-binding proteins.
Western blotting to assess protein interactions and localization.
Cell culture experiments (MCF10A cells) to study phenotypic changes.

Main Results:

Angiomotin-like protein 1 (AMOTL1) and angiomotin-like protein 2 (AMOTL2) were identified as YAP1-binding proteins.
AMOTL1 and AMOTL2 directly interact with YAP1, regulating its translocation between the cytoplasm and nucleus.
This regulation is independent of YAP1 phosphorylation.
AMOTL2 downregulation in MCF10A cells induced epithelial-mesenchymal transition (EMT), similar to YAP1 overexpression.

Conclusions:

A novel mechanism for YAP1 regulation involves direct interaction with AMOTL1 and AMOTL2.
Angiomotin-like proteins modulate YAP1 activity and cellular processes like EMT.
These findings provide new insights into Hippo pathway signaling and organ size control.