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Related Experiment Videos

HLA expression on human ejaculated sperm.

K Ohashi1, F Saji, M Kato

  • 1Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.

American Journal of Reproductive Immunology (New York, N.Y. : 1989)
|May 1, 1990
PubMed
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Human leukocyte antigen (HLA) class II molecules are expressed at low levels on ejaculated sperm, while HLA class I is undetectable. Cytokine treatment did not alter HLA expression on sperm, suggesting limited immune modulation.

Area of Science:

  • Immunology
  • Reproductive Biology
  • Cellular Biology

Background:

  • Human leukocyte antigen (HLA) expression on sperm is crucial for reproductive immunology.
  • Understanding HLA expression on sperm may impact fertility and immune responses.

Purpose of the Study:

  • To investigate the expression of HLA molecules on human ejaculated sperm.
  • To determine the effect of cytokines on HLA expression in sperm.

Main Methods:

  • Cellular binding radioimmunoassay (CB-RIA) was used to detect HLA expression.
  • Sperm samples were purified using Percoll discontinuous gradient.
  • Monoclonal antibodies specific to HLA molecules were employed.
  • K562 cell line was used as a control for cytokine response.

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Main Results:

  • HLA class II (DP, DQ, DR) was detected at low levels on ejaculated sperm.
  • HLA class I was not detected on ejaculated sperm.
  • No individual variation in HLA expression was observed in healthy males.
  • Interferon gamma (IFN-gamma) and/or tumor necrosis factor alpha (TNF-alpha) did not affect HLA expression on sperm.

Conclusions:

  • Human ejaculated sperm express low levels of HLA class II but not HLA class I.
  • Sperm HLA expression is consistent across individuals and resistant to cytokine modulation.