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Updated: Jun 5, 2026
Establishing a Device for Sleep Deprivation in Mice
Published on: September 22, 2023
[Effects of cold stress on blood cell parameters of partial sleep deprivation mice]
Liang Cheng1, Na Wang, Ping Liu
1Hubei Key Laboratory of Genetic Regulation & Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079.
Aim:
To investigate the effect of cold, partial sleep deprivation, partial sleep deprivation plus cold on blood routine parameters and erythrocyte sedimentation rate of mice.
Methods:
Twenty-four Mus musculus mice were divided into four groups (n=6) randomly: (1) control, (2) cold group: mice were treated with (10 +/- 2) degrees C cold stimulation for four hours per day, (3) partial sleep deprivation group: mice were deprived sleep from 18:00 to 9:00 next day, (4) partial sleep deprivation plus cold group: mice were treated with cold stimulation based on partial sleep deprivation. After four days treatment, the mice were sacrificed and the blood was collected to detect the blood routine parameters and erythrocyte sedimentation rate.
Results:
Compared with the control, cold stimulation would increase the contents and proportion of lymphocyte significantly. Partial sleep deprivation would decrease the white blood cell contents, lymphocyte contents and lymphocyte proportion significantly. After treated with cold stimulation plus partial sleep deprivation, the white blood cell and lymphocyte contents decreased and the erythrocyte sedimentation rate increased evidently compared with other three groups.
Conclusion:
Partial sleep deprivation could inhibit immune function of the mice. When the mice were treated with cold stimulation plus partial sleep deprivation, the immune function of the mice would be inhibited further more and at the same time the erythrocyte sedimentation rate increased significantly.
