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Updated: Jan 19, 2026
Development of a Murine Model for Femoral Artery Anastomotic Stenosis
Published on: April 18, 2025
[Change of enzyme activity correlation with nucleic acid metabolism during the vascular intimal hyperplasia]
Lei Nie1, Mei Han, Jin-Kun Wen
1Department of Biochemistry, Institute of Basic Medical Sciences, Hebei Medical University, Shijiazhuang 050017, China.
Aim And Methods:
To determine the relationship between the nuclear envelope nucleoside triphosphatase (EC 3. 6. 1. 15, NTPase) activity and the phenotypic modulation of vascular smooth muscle cell (VSMC), the NTPase activity was detected during restenosis after de-endothelialization in vascular wall. The activities of three enzymes involved in carbohydrate and nucleic acid metabolism were also investigated by spectrophotometry.
Results:
The activity of NTPase increased continuously and associated with the process of intimal thickening. Western blotting showed that expression of SMalpha-actin, as the marker of contractile phenotype of VSMC, decreased continuously. Osteopontin (OPN), the marker of synthetic phenotype of VSMC, was up-regulated during the process. These suggested that intimal injury induced phenotypic modulation of VSMC. The activities of 5'-nucleotidase, adenosine deaminase and succinate dehydrogenase increased and reached their peaks on 7 days after de-endothelialization. The changes of three enzymes were associated with proliferation in VSMC.
Conclusion:
The efflux of mRNA and the changes of enzyme activity involved in carbohydrate or nucleic acid metabolism may be the biochemical basis in the development and progression of restenosis.
