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Updated: Jun 5, 2026
Preparation of Acute Human Hippocampal Slices for Electrophysiological Recordings
Published on: May 7, 2020
[Hippocampal slice - a model in vitro for screening antiepileptic drugs]
1Department of Life Science & Biomedical Science, Zhejiang University, Hangzhou 310027, China.
Aim:
To establish a model in vitro for screening antiepileptic drugs.
Methods:
Perfusing guinea pig hippocampal slice with penicillin offers a stable epileptiform discharge which was tested by two commonly clinically used antiepileptic drugs, phenytoin sodium and phenobarbital sodium at different concentration.
Results:
The experimental result showed that penicillin induced epileptiform discharge in the hippocampal slice and two antiepileptic drugs, phenytoin sodium and phenobarbital sodium inhibition of this discharge induced by penicillin.
Conclusion:
This electrophysiological model has the advantage of simplicity, flexibility and ease of quantitation and has potential value for screen antiepileptic drugs and epilepsy research.
