Related Experiment Video
Updated: Jun 5, 2026

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
Published on: December 30, 2016
Recovering lost magnetization: polarization enhancement in biomolecular NMR
Adrien Favier1, Bernhard Brutscher
1IBS, Institut de Biologie Structurale Jean-Pierre Ebel, 41 rue Jules Horowitz, 38027 Grenoble, France.
Abstract:
Experimental sensitivity remains a major drawback for the application of NMR spectroscopy to fragile and low concentrated biomolecular samples. Here we describe an efficient polarization enhancement mechanism in longitudinal-relaxation enhanced fast-pulsing triple-resonance experiments. By recovering undetectable (1)H polarization originating from longitudinal relaxation during the pulse sequence, the steady-state (15)N polarization becomes enhanced by up to a factor of ~5 with respect to thermal equilibrium yielding significant sensitivity improvements compared to conventional schemes. The benefits of BEST-TROSY experiments at high magnetic field strength are illustrated for various protein applications, but they will be equally useful for other protonated macromolecular systems.
More Related Videos
09:37Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
Published on: February 12, 2019
10:54Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
Published on: February 23, 2016
Related Concept Videos
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)
Atomic Nuclei: Magnetic Resonance
Atomic Nuclei: Nuclear Relaxation Processes
Applications Of NMR In Biology
The...
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)
NMR Spectrometers: Resolution and Error Correction