Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Supercritical Fluid Chromatography01:18

Supercritical Fluid Chromatography

Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advances in the use of DNA aptamers for bioanalysis of antibody-based therapeutics.

Bioanalysis·2026
Same author

Atypical endo-β-1,4-mannanases are necessary for normal glucomannan synthesis in Arabidopsis.

Plant physiology·2026
Same author

Development of a facile and novel strategy for N-glycan analysis: Stable isotope labeling via Endo‑CC N180H‑catalyzed transglycosylation for quantitative glycomics.

Journal of pharmaceutical and biomedical analysis·2026
Same author

Foxp3 and BATF cooperatively direct cis-regulatory programs and gene expression for effector Treg cell differentiation.

Immunity·2026
Same author

Poly(2-ethyl-2-oxazoline)-Conjugated Porcine Serum Albumin as a Veterinary Albumin Therapeutic.

ACS applied bio materials·2026
Same author

Enantioselective Determination of Carnitine Enantiomers in Food and Supplement Samples by Chiral Liquid Chromatography-Tandem Mass Spectrometry.

Mass spectrometry (Tokyo, Japan)·2026

Related Experiment Video

Updated: Jun 5, 2026

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
14:42

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

Highly sensitive and selective derivatization-LC method for biomolecules based on fluorescence interactions and

Kenichiro Todoroki1, Hideyuki Yoshida, Tadashi Hayama

  • 1Faculty of Pharmaceutical Sciences, Fukuoka University, Nanakuma, Johnan, Fukuoka 814-0180, Japan.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|December 31, 2010
PubMed
Summary

This review highlights advanced fluorescence derivatization liquid chromatography (LC) methods for sensitive biological compound analysis. New detection-selective and separation-selective techniques enhance analytical performance.

More Related Videos

Detection of CD40 Protein-Umbelliferone Interaction via Differential Scanning Fluorescence
05:30

Detection of CD40 Protein-Umbelliferone Interaction via Differential Scanning Fluorescence

Published on: March 1, 2024

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules
10:23

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules

Published on: April 25, 2025

Related Experiment Videos

Last Updated: Jun 5, 2026

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
14:42

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

Detection of CD40 Protein-Umbelliferone Interaction via Differential Scanning Fluorescence
05:30

Detection of CD40 Protein-Umbelliferone Interaction via Differential Scanning Fluorescence

Published on: March 1, 2024

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules
10:23

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules

Published on: April 25, 2025

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Chromatography

Background:

  • Liquid chromatography (LC) is crucial for analyzing biological compounds.
  • High sensitivity and selectivity are essential for accurate biological analyses.
  • Fluorescence derivatization enhances LC method performance.

Purpose of the Study:

  • To review novel fluorescence derivatization LC analysis methods.
  • To categorize new methods based on their selectivity.
  • To provide an overview of advanced analytical techniques.

Main Methods:

  • Detection-selective derivatization: excimer fluorescence and fluorescence resonance energy transfer (FRET).
  • Separation-selective derivatization: fluorous derivatization, F-trap fluorescence derivatization, and fluorous scavenging derivatization (FSD).
  • Utilizing fluorous separation techniques for enhanced analyte isolation.

Main Results:

  • Introduction of novel detection-selective fluorescence derivatization techniques.
  • Presentation of innovative separation-selective methods employing fluorous chemistry.
  • Demonstration of enhanced sensitivity and selectivity in biological compound analysis.

Conclusions:

  • Fluorescence derivatization LC offers powerful tools for sensitive and selective biological analysis.
  • New detection- and separation-selective methods expand analytical capabilities.
  • These advanced techniques improve the analysis of complex biological matrices.