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Related Experiment Video

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The Isolation of Flowing Mesenteric Lymph in Mice to Quantify In Vivo Kinetics of Dietary Lipid Absorption and Chylomicron Secretion
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Proteomic analysis of human mesenteric lymph.

Monika Dzieciatkowska1, Max V Wohlauer, Ernest E Moore

  • 1Department of Biochemistry and Molecular Genetics, University of Colorado, Denver, CO, USA.

Shock (Augusta, Ga.)
|December 31, 2010
PubMed
Summary

Mesenteric lymph plays a key role in distant organ injury following gut ischemia/reperfusion. Human mesenteric lymph activates neutrophils and contains proteins linked to tissue damage and shock, supporting its role in systemic inflammation.

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Area of Science:

  • Physiology
  • Immunology
  • Proteomics

Background:

  • Animal studies identify mesenteric lymph as a link between gut ischemia/reperfusion and distant organ injury.
  • Human trauma and transplant services offer a unique setting to study mesenteric lymph in relevant clinical conditions.

Purpose of the Study:

  • To assess the bioactivity and proteomic profile of human mesenteric lymph.
  • To determine if human mesenteric lymph activates neutrophils similarly to animal models.
  • To investigate the correlation between lymph composition and clinical factors like tissue injury and shock.

Main Methods:

  • Mesenteric lymph was collected from 11 patients undergoing spine reconstruction or organ donation.
  • Human mesenteric lymph was tested for its ability to activate human neutrophils in vitro.
  • Label-free proteomic analysis was performed on lymph samples to identify proteins.

Main Results:

  • Human mesenteric lymph primed human polymorphonuclear neutrophils, mirroring findings in animal studies.
  • Proteomic analysis identified 477 proteins, including serum proteins, hemolysis markers, extracellular matrix components, and tissue damage markers.
  • Tissue injury and shock strongly correlated with bioactive lymph production. Red blood cell hemolysis products correlated with lymph bioactivity, while immunoglobulins showed a negative correlation.

Conclusions:

  • Human mesenteric lymph contains bioactive components that activate neutrophils.
  • The identified proteins and their correlation with clinical factors support the role of mesenteric lymph in systemic inflammation and multiple organ failure post-shock.
  • Further research is needed to explore the diagnostic potential of these proteins in multiple organ failure.