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Updated: Jul 11, 2026
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Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture
Published on: May 26, 2016
Tissue culture in hollow-fibre systems: implications for downstream processing and stability analysis
1Euroclone B.V., Plesmanlaan 125, NL-1066 CX Amsterdam, The Netherlands.
Developments in Biological Standardization
|January 1, 1990
Summary
Hollow-fibre bioreactors enhance monoclonal antibody (MAb) production by enabling lower serum concentrations compared to stirred-tank systems. This method yields higher MAb concentration and purity for clinical applications.
Area of Science:
- Biotechnology
- Cell Culture Engineering
Background:
- Murine hybridoma cells require specific culture conditions.
- Optimizing monoclonal antibody (MAb) production is crucial for clinical applications.
Purpose of the Study:
- To compare MAb production efficiency between hollow-fibre and stirred-tank bioreactor systems.
- To evaluate the impact of reduced serum concentration on MAb yield and purity.
Main Methods:
- Culturing hybridoma cells in hollow-fibre and stirred-tank perfusion systems.
- Gradually decreasing serum content in the culture medium.
- Analyzing MAb concentration, production rate, and protein purity in harvested samples.
Main Results:
- Hollow-fibre bioreactors achieved significantly higher MAb concentrations than stirred-tank systems.
- Cell production rates in hollow-fibre systems remained stable at 0.5% serum, unlike stirred-tank systems requiring 2%.
- The ratio of MAb to serum proteins was considerably higher in the hollow-fibre system's harvest.
Conclusions:
- Hollow-fibre bioreactors offer superior performance for MAb production, especially under low-serum conditions.
- This technology enhances MAb purity and yield, making it suitable for clinical applications.
- Further investigation into cell mass stability within hollow-fibre systems is warranted.
