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Published on: July 22, 2012
Human rotavirus genotypes detection among hospitalized children, a study in Tehran, Iran
Shahrzad Modaress1, Ali Akbar Rahbarimanesh, Rosita Edalat
1Department of Virology, Pasteur Institute, Tehran, Iran. smodaress@pasteur.ir
Insights
The G1P[8] rotavirus genotype was highly prevalent in children with gastroenteritis in Tehran, Iran. This finding is crucial for developing effective rotavirus vaccines in the region.
Area of Science:
- Virology
- Pediatrics
- Public Health
Background:
- Group A rotaviruses are a leading cause of acute gastroenteritis in children globally.
- Common human rotavirus genotypes include G1-G4, G9, P[4], and P[8].
- The G1P[8] rotavirus genotype is frequently identified in many countries.
Purpose of the Study:
- To investigate the genotypic diversity of group A rotaviruses in children under five years of age in Tehran, Iran.
- To identify the predominant rotavirus genotypes causing gastroenteritis in the study population.
Main Methods:
- Collected 700 stool specimens from children presenting with dehydration and diarrhea.
- Utilized dsRNA-PAGE technique for rotavirus detection.
- Performed G and P typing using semi-nested multiplex RT-PCR.
Main Results:
- Rotaviruses were detected in 19% of the analyzed stool samples.
- The predominant G genotype was G1 (76.3%), followed by G4 (11.5%).
- The most common P genotype was P[8] (66.4%), followed by P[4] (9.2%).
- The G1P[8] genotype was the most frequent mixed type, found in 53.4% of positive samples.
Conclusions:
- The G1P[8] genotype was highly prevalent, causing childhood gastroenteritis in Tehran, Iran.
- Identified rotavirus G and P genotypes showed similarities to strains found in other Asian countries.
- The study's findings are valuable for future rotavirus vaccine development and formulation in Iran.
Background:
Human rotavirus genotypes G1-G4, G9, P[4] and P[8] are major worldwide causes of acute gastroenteritis in children. Rotavirus genotype G1P[8] is predominant in many countries. In this study, the genotypic diversity of group A rotaviruses were detected in children <5 years of age who were treated for dehydration and diarrhea in Tehran, Iran from October 2004 to September 2008.
Methods:
A total of 700 stool specimens were collected from children and assessed for the presence of rotaviruses by the dsRNA-PAGE technique. G and P typing of the positive samples were performed by semi-nested multiplex RT-PCR.
Results:
Rotaviruses were isolated in 19% of samples. A total of 14 rotavirus dsRNA different electrophoretypes were detected. The predominant genotype was G1 (76.3%), followed by G4 (11.5%), G8 (0.8%), P[4] (9.2%) and P[8] (66.4%), respectively. In mixed type samples, the majority were of genotype G1P[8] (53.4%), followed by G1P[4] (9.2%) and G4P[8] (4.6%). Mixed types consisted of 3.1% of the total sample followed by G1G2/-P (1.5%), G1G4P[4] (0.8%) and G1G4P[8] (0.8%).
Conclusion:
In this study, a high prevalence of the G1P[8] genotype was determined to be the cause of childhood gastroenteritis in Tehran, Iran. The sequence of G and P genotypes showed high levels of similarity to strains from other Asian countries. Our data will be useful for future vaccine formulation in Iran.
