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Updated: Jun 5, 2026
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Isolation of Umbilical Cord-Derived Mesenchymal Stem Cells with High Yields and Low Damage
Published on: July 5, 2024
[Two different techniques for volume blood reduction obtained from umbilical cord]
Gamaliel Benítez-Arvizu1, Samantha Martínez, Ángel Guerra
1Banco Central de Sangre, Centro Médico Nacional La Raza, Instituto Mexicano del Seguro Social, Distrito Federal, México. gamardoc@yahoo.com.mx
Revista Medica Del Instituto Mexicano Del Seguro Social
|January 4, 2011
Summary
Two umbilical cord blood (UCB) volume reduction techniques were compared. Both methods effectively preserved cell quality, quantity, and functionality for cryopreservation, with minor viability differences.
Area of Science:
- Hematology
- Cellular Biology
- Biotechnology
Background:
- Umbilical cord blood (UCB) requires volume reduction before cryopreservation.
- Maintaining cell quality, quantity, and functionality during UCB processing is crucial.
Purpose of the Study:
- To compare the performance of semiautomatic and automatic systems for UCB volume reduction.
- To evaluate cell recovery and viability after different UCB volume reduction techniques.
Main Methods:
- Comparison of semiautomatic and automatic UCB volume reduction systems.
- Measurement of total nucleated cell (TNC) counts and viability before and after reduction.
- Assessment of CD34+ cell counts post-volume reduction.
Main Results:
- Seventy UCB units were processed (33 semiautomatic, 37 automatic).
- Both techniques yielded similar recovered volumes and CD34+ cell counts.
- A slight difference in viability was observed, with the automatic system showing 1% higher viability.
Conclusions:
- Both semiautomatic and automatic techniques are suitable for UCB volume reduction.
- These methods effectively prepare UCB for cryopreservation while preserving key cell parameters.
