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Fructose formation in stored blood
M Guppy1, R Sabaratnam, S Devadason
1Biochemistry Department, University of W.A., Nedlands, Australia.
Biochemistry International
|January 1, 1990
Summary
High glucose in donor blood collection may deplete cell antioxidants via the polyol pathway. However, elevated fructose found in blood and anticoagulant is formed during autoclaving, not by this pathway.
Area of Science:
- Biochemistry
- Hematology
- Biomedical Science
Background:
- High glucose concentrations in donor blood collection and storage can potentially induce oxidative stress.
- The fructose-forming polyol pathway (Reactions I and II) might deplete NADPH and glutathione (GSH), compromising red blood cell integrity.
Purpose of the Study:
- To investigate the source of elevated fructose levels in donor blood and anticoagulant solutions.
- To determine if the polyol pathway contributes to fructose formation and potential oxidative stress in stored blood.
Main Methods:
- Analysis of fructose levels in red blood cells and plasma from donor blood collected in CP2D.
- Assessment of fructose origin, distinguishing between polyol pathway activity and autoclaving processes.
- Quantification of fructose in CP2D anticoagulant and autoclaved glucose samples.
Main Results:
- High fructose levels were detected in both red blood cells and plasma of donor blood collected in CP2D (258 mM glucose).
- Elevated fructose was not a product of the polyol pathway.
- Autoclaving of glucose was identified as the source of high fructose concentrations found in the anticoagulant and donor red cells.
Conclusions:
- The observed high fructose levels in donor blood are primarily due to the autoclaving process used for glucose preparation, not the polyol pathway.
- The polyol pathway's potential role in depleting antioxidants under these conditions requires further investigation, as autoclaving is the main source of fructose.