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Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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Updated: Jun 5, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Holographic opto-fluidic microscopy.

Waheb Bishara1, Hongying Zhu, Aydogan Ozcan

  • 1Electrical Engineering Department, University of California, Los Angeles, CA 90095, USA. bishara@ucla.edu

Optics Express
|January 4, 2011
PubMed
Summary
This summary is machine-generated.

We developed Holographic Opto-fluidic Microscopy (HOM), a new lensless imaging technique. HOM integrates microscopy into microfluidic devices for high-resolution imaging of micro-organisms and particles without complex setups.

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Area of Science:

  • Optics and Photonics
  • Microfluidics
  • Biotechnology

Background:

  • Microfluidics enables advanced control and analysis of micro-scale objects.
  • Microfluidics integrated with optics has led to reconfigurable optical devices.
  • On-chip microscopy is desirable for integration with microfluidic systems.

Purpose of the Study:

  • To introduce a novel opto-fluidic microscopy modality: Holographic Opto-fluidic Microscopy (HOM).
  • To enable high-resolution imaging within microfluidic channels.
  • To complement miniaturization trends in microfluidic devices.

Main Methods:

  • Utilized lensless holographic imaging based on partially coherent in-line holography.
  • Employed pixel super-resolution for enhanced image quality.
  • Demonstrated imaging of micro-organisms (C. elegans, Giardia lamblia) and particles (Mulberry pollen).

Main Results:

  • Achieved high-resolution amplitude and phase imaging of flowing micro-objects.
  • Successfully imaged C. elegans, Giardia lamblia, and Mulberry pollen within microfluidic channels.
  • HOM requires no complicated fabrication or precise alignment.

Conclusions:

  • HOM provides a versatile and integrated microscopy solution for microfluidic devices.
  • The technique simplifies imaging processes, removing the need for uniform flow.
  • HOM facilitates the incorporation of advanced imaging capabilities into existing microfluidic platforms.