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Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
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IMAPlate based miniature, high sensitive, rapid screening method for detecting bioengineered, secreted lipase
Michel A Sciotti1, Guo Jun Chen, Daniel Gygax
1Hochschule für Life Sciences, Fachhochschule Nordwestschweiz Grandenstrasse 40, Muttenz.
Chimia
|January 5, 2011
Abstract:
A spectrophotometric assay based on a miniaturized 96-well plate device (IMAPlate) enables a rapid and simple screening of bioengineered recombinant lipases expressed and secreted by Saccharomyces cerevisiae. Starting from a colony, the test delivers a quantitative estimation of enzymatic activity titer in 24 h or less with manual high throughput performances.
