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Updated: Jun 5, 2026

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy
Published on: July 10, 2019
Ferrule-top atomic force microscope.
1Faculty of Sciences, Department of Physics and Astronomy and LaserLaB, Vrije Universiteit, Amsterdam, The Netherlands.
Ferrule-top cantilevers enable ultra-compact atomic force microscopy (AFM) for imaging in air and liquids. These all-optical devices offer sensitivity comparable to commercial AFMs and are easy to handle.
Area of Science:
- Microscopy and Nanotechnology
- Optical Physics
- Materials Science
Background:
- Ferrule-top cantilevers are all-optical miniaturized devices designed for use in liquids and harsh environments.
- They are fabricated by shaping the end of a ferruled fiber into a mechanical beam, enabling optical detection of deflections.
Purpose of the Study:
- To demonstrate the utility of ferrule-top cantilevers for developing ultra-compact atomic force microscopes (AFMs).
- To evaluate their performance in contact mode imaging in both air and liquid environments.
Main Methods:
- Fabrication of ferrule-top cantilevers by carving ferruled fiber ends into mechanical beams.
- Utilizing light coupled through the fiber for detecting cantilever deflections.
- Performing contact mode imaging in air and liquids using the developed AFM system.
Main Results:
- Ferrule-top cantilevers facilitate the development of ultra-compact AFMs.
- The developed AFM system achieves sensitivity comparable to commercial AFMs.
- The probes demonstrate ease of handling and require no alignment procedures.
Conclusions:
- Ferrule-top cantilevers are suitable for creating highly sensitive, compact AFM systems.
- Their robustness and ease of use expand AFM applications beyond traditional laboratory settings.
- These devices offer a promising platform for advanced imaging in diverse environments.
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