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Related Experiment Videos

Correlation between poly(U) misreading and poly(dT) translation efficiency in E coli cell-free systems.

A P Potapov1, I S Groisman, A V El'skaya

  • 1Institute of Molecular Biology and Genetics, Ukrainian SSR Academy of Sciences, Kiev.

Biochimie
|May 1, 1990
PubMed
Summary

Poly(U) misreading positively correlates with poly(dT) translation efficiency in E. coli. Ribosomal protein S12 mutants and antibiotics like neomycin affect both processes, supporting a codon-anticodon stabilization hypothesis.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Ribosomal protein S12 plays a critical role in translation accuracy.
  • Aminoglycoside antibiotics are known to induce ribosomal frameshifting and misreading.
  • The translation of poly(dT) sequences is poorly understood.

Purpose of the Study:

  • To investigate the relationship between poly(U) misreading and poly(dT) translation efficiency.
  • To explore the role of ribosomal protein S12 in these processes.
  • To determine the effect of aminoglycoside antibiotics and Mg2+ ions on poly(dT) translation.

Main Methods:

  • Cell-free translation systems using wild-type E. coli and S12 mutants.
  • Analysis of poly(U) misreading and poly(dT) translation efficiency.

Related Experiment Videos

  • Treatment with various aminoglycoside antibiotics (neomycin, kanamycin, streptomycin) and Mg2+ ions.

    • Main Results:

    • A positive correlation was observed between poly(U) misreading and poly(dT) translation efficiency.
    • Factors promoting poly(U) misreading, including antibiotics and Mg2+ ions, also stimulated poly(dT) translation.
    • S12 mutant ribosomes exhibited reduced poly(U) misreading and less efficient poly(dT) decoding.
    • The order of antibiotic effect was neomycin > kanamycin > streptomycin for both poly(U) and poly(dT) translation.

    Conclusions:

    • The findings support the hypothesis of stereospecific stabilization of codon-anticodon complexes by the ribosome decoding center.
    • Ribosomal protein S12 influences both translation accuracy and the efficiency of poly(dT) translation.
    • Aminoglycoside antibiotics modulate translation fidelity and efficiency in a dose-dependent manner.