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Correlation between poly(U) misreading and poly(dT) translation efficiency in E coli cell-free systems
A P Potapov1, I S Groisman, A V El'skaya
1Institute of Molecular Biology and Genetics, Ukrainian SSR Academy of Sciences, Kiev.
Abstract:
A positive correlation between poly(U) misreading and efficiency of poly(dT) translation has been revealed in cell-free systems from wild-type E coli and streptomycin--resistant mutants with altered ribosomal protein S12. Different factors promoting misreading of poly(U) such as aminoglycoside antibiotics and Mg2+ ions also stimulate poly(dT) translation. The effect of the antibiotics on poly(U) translation efficiency and misreading as well as on poly(dT) decoding is characterised by the same order: neomycin greater than kanamycin greater than streptomycin. S12 mutants ribosomes are less erroneous in poly(U) translation and less efficient in poly(dT) decoding. The data obtained are in good agreement with the hypothesis of stereospecific stabilization of codon-anticodon complexes by the ribosome decoding centre.
Insights
Poly(U) misreading positively correlates with poly(dT) translation efficiency in E. coli. Ribosomal protein S12 mutants and antibiotics like neomycin affect both processes, supporting a codon-anticodon stabilization hypothesis.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Ribosomal protein S12 plays a critical role in translation accuracy.
- Aminoglycoside antibiotics are known to induce ribosomal frameshifting and misreading.
- The translation of poly(dT) sequences is poorly understood.
Purpose of the Study:
- To investigate the relationship between poly(U) misreading and poly(dT) translation efficiency.
- To explore the role of ribosomal protein S12 in these processes.
- To determine the effect of aminoglycoside antibiotics and Mg2+ ions on poly(dT) translation.
Main Methods:
- Cell-free translation systems using wild-type E. coli and S12 mutants.
- Analysis of poly(U) misreading and poly(dT) translation efficiency.
- Treatment with various aminoglycoside antibiotics (neomycin, kanamycin, streptomycin) and Mg2+ ions.
Main Results:
- A positive correlation was observed between poly(U) misreading and poly(dT) translation efficiency.
- Factors promoting poly(U) misreading, including antibiotics and Mg2+ ions, also stimulated poly(dT) translation.
- S12 mutant ribosomes exhibited reduced poly(U) misreading and less efficient poly(dT) decoding.
- The order of antibiotic effect was neomycin > kanamycin > streptomycin for both poly(U) and poly(dT) translation.
Conclusions:
- The findings support the hypothesis of stereospecific stabilization of codon-anticodon complexes by the ribosome decoding center.
- Ribosomal protein S12 influences both translation accuracy and the efficiency of poly(dT) translation.
- Aminoglycoside antibiotics modulate translation fidelity and efficiency in a dose-dependent manner.