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Related Experiment Video

Updated: Jun 5, 2026

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Resonant waveguide grating imager for live cell sensing.

Ann M Ferrie1, Qi Wu, Ye Fang

  • 1Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, New York 14831, USA.

Applied Physics Letters
|January 5, 2011
PubMed
Summary
This summary is machine-generated.

We developed a resonant waveguide grating imager for rapid, high-throughput live-cell screening. This biosensor imager captures 384-well plates in seconds with high spatial resolution, enabling robust cell response analysis.

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Area of Science:

  • Biophotonics
  • Cellular Imaging
  • High-Throughput Screening

Background:

  • Live-cell imaging is crucial for understanding cellular dynamics.
  • Existing high-throughput screening methods often lack speed or spatial resolution.
  • Biosensor-based assays require sensitive detection of cellular responses.

Purpose of the Study:

  • To develop and characterize a novel resonant waveguide grating imager for live-cell screening.
  • To assess the imager's performance in terms of speed, resolution, and tolerance to experimental variability.
  • To demonstrate the utility of the imager for quantifying cell confluency and responses.

Main Methods:

  • Utilized a resonant waveguide grating imager for snapshot imaging of 384-well microtiter plates.
  • Achieved a time resolution of approximately 3 seconds and a spatial resolution of 80 μm.
  • Investigated imager tolerance to variations in plate configuration and cell confluency.
  • Correlated resonant wavelength shifts with cell confluency and cellular responses.
  • Developed a data filtration protocol to enhance assay quality.

Main Results:

  • The imager successfully captured images of all biosensors in a 384-well plate within seconds.
  • Demonstrated high tolerance to variability in plate setup and cell confluency.
  • Observed a strong correlation between resonant wavelength shifts and cell confluency.
  • Showcased the effectiveness of the data filtration protocol for improving assay quality, especially with partially confluent cells.

Conclusions:

  • The resonant waveguide grating imager offers a rapid and high-throughput solution for live-cell screening.
  • The imager's performance is robust and adaptable to common experimental variations.
  • Wavelength shift analysis provides a quantitative measure of cell confluency and response, enhancing biological insights.