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Updated: Jun 5, 2026

Neisseria meningitidis Infection of Induced Pluripotent Stem-Cell Derived Brain Endothelial Cells
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[Study on gene expression pattern in 16HBE cells treated with NiS].

Xiaonong Bin1, Jiachun Lv, Min Tan

  • 1Institute for Chemical Carcinogenesis, Guangzhou Medical College, Guangzhou, Guangdong 510182, P.R.China.

Zhongguo Fei AI Za Zhi = Chinese Journal of Lung Cancer
|January 6, 2011
PubMed
Summary

Nickel sulfide (NiS) exposure transforms human bronchial epithelial cells (16HBE), altering gene expression. This study identified 151 differentially expressed genes, revealing potential molecular mechanisms of NiS carcinogenicity.

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Area of Science:

  • Environmental Toxicology
  • Molecular Biology
  • Genomics

Context:

  • Nickel sulfide (NiS) is recognized for its malignant transforming activity and carcinogenicity.
  • Human bronchial epithelial cells (16HBE) are susceptible to NiS-induced cellular transformation.
  • The molecular mechanisms underlying NiS carcinogenicity may involve gene mutations and altered transcription factor expression.

Purpose:

  • To screen differentially expressed genes in 16HBE cells transformed by NiS.
  • To identify genes and pathways affected by NiS exposure in bronchial epithelial cells.

Summary:

  • A cDNA microarray analysis was performed on 16HBE cells and NiS-treated 16HBE cells (NiS-16HBE).
  • Total RNA was extracted, converted to cDNA probes, and hybridized to a microarray containing 4000 human genes.

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  • Analysis revealed 151 differentially expressed genes, with 70 down-regulated and 81 up-regulated.

    • Impact:

    • Identifies key genes and biological processes, including stress response, immune function, DNA repair, metabolism, and oncogenes/tumor suppressors, potentially involved in NiS-induced cell transformation.
    • Provides a foundation for understanding the molecular basis of NiS carcinogenicity.
    • Highlights potential targets for future research into prevention and treatment strategies.