Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Achieving Effective Batch-to-Batch Error Correction through Suppression Correction and Dual MSTUS Normalization.

Analytical chemistry·2026
Same author

Urinary biochemical ecology reveals microbiome-metabolite interactions and metabolic markers of recurrent urinary tract infection.

NPJ biofilms and microbiomes·2025
Same author

Urinary biochemical ecology reveals microbiome-metabolite interactions and metabolic markers of recurrent urinary tract infection.

bioRxiv : the preprint server for biology·2024
Same author

New Processes for Ionizing Nonvolatile Compounds in Mass Spectrometry: The Road of Discovery to Current State-of-the-Art.

Journal of the American Society for Mass Spectrometry·2024
Same author

Direct sub-atmospheric pressure ionization mass spectrometry: Evaporation/sublimation-driven ionization is amazing, fundamentally, and practically.

Journal of mass spectrometry : JMS·2024
Same author

Phenolic composition and bioactivity of <i>Ribes magellanicum</i> fruits from southern Patagonia.

Heliyon·2024
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Jun 5, 2026

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)

Published on: March 14, 2013

LC-MS-based metabolomics.

Sunil Bajad1, Vladimir Shulaev

  • 1Sutro Biopharma Inc., South San Francisco, CA, USA. sbajad@yahoo.com

Methods in Molecular Biology (Clifton, N.J.)
|January 6, 2011
PubMed
Summary
This summary is machine-generated.

This study presents a simple hydrophilic interaction chromatography (HILIC) liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for analyzing polar metabolites. The validated technique enables relative quantitation of hundreds of polar compounds across diverse biological samples.

More Related Videos

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

Published on: May 20, 2013

Related Experiment Videos

Last Updated: Jun 5, 2026

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)

Published on: March 14, 2013

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

Published on: May 20, 2013

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Metabolomics

Background:

  • Metabolomics requires analyzing numerous polar metabolites, posing significant chromatographic challenges.
  • Hydrophilic interaction chromatography (HILIC) has emerged as a key technique for polar molecule analysis.

Purpose of the Study:

  • To develop and validate a simple HILIC LC-MS/MS method for targeted relative quantitation of polar metabolites.
  • To establish a versatile method applicable to various biological sample types.

Main Methods:

  • Utilized hydrophilic interaction chromatography (HILIC) with an aminopropyl column.
  • Employed liquid chromatography-tandem mass spectrometry (LC-MS/MS) for targeted multiple reaction monitoring (MRM).
  • Used acetonitrile as the weak solvent and ammonium acetate buffer as the strong solvent, avoiding ion pairing reagents.

Main Results:

  • Successfully developed a simple HILIC LC-MS/MS method for relative quantitation of hundreds of polar metabolites.
  • The method demonstrated suitability for both positive and negative ionization modes.
  • Validated method applied effectively to diverse samples including bacteria, yeast, plants, human body fluids, and cell cultures.

Conclusions:

  • The presented HILIC LC-MS/MS method offers a robust and straightforward approach for polar metabolite profiling.
  • The method's versatility and validation make it valuable for comparative metabolomics studies across various biological systems.