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[Effects of genistein on proliferation and differentiation of osteoblast]
Yue-hong Zhang1, Lan-xing Gao, Hong Jin
1Institue of Health and Environmental Medicine, Tianjin 300050, China.
Aim:
To study the effects of genistein on proliferation and differentiation of osteoblasts in neonatal rat calvaria cultures.
Methods:
Osteoblasts were isolated from neonatal rat calvaria through trypsin and collagenase digestion, and cultured in the presence of different doses of genistein (10(-5) mol/L, 10(-6) mol/L and 10(-7) mol/L). The proliferation and DNA and collagen synthesis of osteoblasts were assayed by MTT method and 3H-TdR and 3H-proline incorporation. The activity of ALP were measured by ALP assay kit.
Results:
Genistein significantly increased osteoblast 3H-TdR and 3H-proline incorporation and MTT, 10(-6) mol/L genistein increased ALP activity.
Conclusion:
Genistein increased osteoblast DNA and collagen synthesis in neonatal rat calvaria cultures, and promoted osteoblast proliferation and differentiation.
