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Published on: July 17, 2011
[Properties of single L-type Ca2+ channels in hypothalamic neurons of newborn rats]
1Department of High Temperature, First Military Medical University, Guangzhou 510515, China.
Aim:
To investigate the characteristics of single L-type Ca2+ channels in hypothalamic neurons.
Methods:
The cell-attached patch-clamp technique and acute cell isolation technique were used.
Results:
(1) L-type Ca2+ channels opened throughout an 768 ms long voltage pulse. (2) The averaged open-time constant was 0.28 ms(tau0), two closed-time constants were 2.91 (tau(c1)) and 53.22 ms(tau(c2)). (3) The conductance was (29.5 +/- 3.1) pS and (4) Bay K 8644 increased the open probability by prolonging the mean open time to 1.61 ms.
Conclusion:
L-type Ca2+ channel existed in hypothalamic neurons, with the similar properties to Ca2+ currents as previously reported, but showed its own peculiarity.
