Related Experiment Video
Updated: Jun 5, 2026

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
Published on: February 25, 2016
[Endotoxin-induced generation of peroxynitrite in bovine pulmonary arterial endothelial cells]
1Department of Pathophysiology, Hebei Medical University, Shijiazhuang 050017, China.
Aim:
To explore the significance of bovine pulmonary arterial endothelial cell (BPAEC) to produce peroxynitrite (ONOO-).
Methods:
The contents of nitrotyrosine (NT), a marker of ONOO- generation in vivo,were quantitatively measured with flow cytometric and immunofluorescent technique in cultured BPAEC induced by lipopolysaccharide (LPS), a major component of bacterial endotoxin. In addition, effect of ONOO- on micromorphologic features of BPAEC was observed.
Results:
LPS caused significant increase of NT in BPAEC in a dose-dependent manner, which was in part reversed by aminoguanidine, a selective inhibitor of inducible nitric oxide synthase. Exposure of BPAEC to exogenous ONOO- resulted in the cellular retro-contraction with diminished cellular body and increased width of cellular junction.
Conclusion:
LPS may induce the ONOO- formation of BPAEC. Endothelial cell-derived ONOO- may mediate the deleterious effect of LPS on BPAEC per se.
Related Concept Videos
Inhalation Anthrax
Nitric Oxide Signaling Pathway

