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Updated: Jun 5, 2026
Isolating Bronchial Epithelial Cells from Resected Lung Tissue for Biobanking and Establishing Well-Differentiated Air-Liquid Interface Cultures
Published on: May 26, 2023
[Resisting injury and protective effect of fibronectin on bronchial epithelial cells]
1Department of Physiology, Xiangya Medical School, Central-South University, Changsha 410078, China.
Aim And Methods:
To test the hypothesis that activation of integrin molecules play a role on protecting bronchial epithelial cells (BEC) from oxidant injury. Based on a cell-injury model made by ozone (1.5 x 10(-6)) exposure, the present study investigated the protective effect of fibronectin (Fn), a kind of ligand of integrin, and it's specific sequence Arg-Gly-Asp (RGD peptide) which is a recognizable domain for integrin on BEC. 3H release, LDH and MDA were measured as indexes for damage.
Results:
(1) Ozone exposure induced a significant increase of 3H release and LDH release from BEC and elevation of MDA. (2) Pretreatment with Fn or RGD before ozone exposure markedly attenuated the release of 3H, LDH and MDA, and the effects of Fn were reversed by W7, a calmodulin inhibitor. (3) Either Fn or RGD up-regulated activity of catalase in BEC and promoted GSH synthesis in BEC.
Conclusion:
These suggests that the binding of integrin with Fn improve the antioxidant ability of BEC and lighten the damage induced by oxidant attack. The protective mechanism of Fn-integrin binding may be related with calmodulin.
