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[Effect of 17beta-estradiol on phosphorylcholine cytidylyltransferase activity from cultured rat lung explants]
Cha-Xiang Guan1, Fu-Wen Zhou, Zi-Qiang Luo
1Department of Physiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Aim:
To investigate the influence and mechanisms of 17beta-estradiol on the CTP: phosphorylcholine cytidylyltransferase (CCT) activity from cultured lung explants without serum.
Methods:
We detected the amount of [M-14C] choline incorporation into phosphatidylcholine so as to reflect CCT activity by liquid scintillation.
Results:
(1) 17beta-estradiol increased the CCT activity in dose-dependence and time-dependence. (2) Both the protein kinase C inhibitor H-7 and calmodulin antagonist W-7 abolished the stimulatory effect of 17beta-estradiol (3 x 10(-6) mol/L) on the CCT activity.
Conclusion:
17beta-estradiol can increase CCT activity in cultured lung explants, its mechanism is related to protein kinase C and calmodulin.
