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Published on: November 9, 2017
Multiplex cytokine profiling in patients with sepsis
Simona Mera1, Doina Tatulescu, Cristina Cismaru
1Clinic of Infectious Diseases, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania. simona.mera@yahoo.com
Summary
Multiplex cytokine analysis in sepsis identified specific inflammatory markers like interleukin-8 (IL-8) that predict patient outcomes. Early detection of elevated IL-8 and monocyte chemotactic protein-1 (MCP-1) indicates higher mortality risk in sepsis patients.
Area of Science:
- Clinical Medicine
- Immunology
- Biochemistry
Background:
- Sepsis diagnosis and monitoring require understanding its complex inflammatory mediators.
- Identifying key biomolecules can improve patient outcomes and therapeutic strategies.
Purpose of the Study:
- To evaluate the simultaneous measurement of 17 cytokines using multiplex assays for sepsis diagnosis and monitoring.
- To correlate cytokine levels with sepsis severity, septic shock, and patient survival.
Main Methods:
- Simultaneously measured 17 cytokines in 30 sepsis patients over 7 days using multiplex technique.
- Analyzed cytokine levels in relation to Sepsis-related Organ Failure Assessment (SOFA) score, septic shock, and survival.
- Utilized multivariate logistic regression to identify predictive cytokine markers for mortality.
Main Results:
- Elevated monocyte chemotactic protein-1 (MCP-1), macrophage inflammatory protein (MIP)-1β, and interleukin (IL)-8 correlated with SOFA score.
- Lower initial levels of MIP-1β and granulocyte macrophage colony-stimulating factor were observed in patients with septic shock.
- Higher early IL-8 and MCP-1 levels were associated with non-survival; IL-8 was the most significant predictor of fatal outcome.
- Non-survivors showed persistent increases in IL-1β, IL-6, IL-8, IL-12, interferon-γ, granulocyte colony-stimulating factor, and tumor necrosis factor-α.
Conclusions:
- Simultaneous cytokine profiling in sepsis offers insights into complex inflammatory patterns.
- Specific cytokines, particularly IL-8, are valuable biomarkers for predicting sepsis severity and mortality.
- Multiplex cytokine analysis holds potential for improved sepsis management and targeted therapies.
