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Updated: Jun 5, 2026
Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells
Published on: February 15, 2019
[Construction of eukaryotic expression vector of human CD34 and establishment of stably transfected cell line]
Lin Shi1, Hao Qu, Hong-qin Liu
1Union Stem Cell & Gene Engineering Co., Ltd., Tianjin 300384, China. shilin0609@126.com
Aim:
To construct eukaryotic expression vector of human CD34 and transfect it to 3T3 cells so as to establish stably transfected 3T3 cell line.
Methods:
The RNA was extracted from KG1a. CD34 gene was amplified by RT-PCR. With the double-enzyme digestion, CD34 gene was cloned into pCI-neo eukaryotic expression vector, yielding pCI-CD34. The pCI-CD34 was transfected into 3T3 cell by electroporator. Stably transfected 3T3 cell line was established, and the CD34 expression in the transfected cells was detected by RT-PCR and FACS.
Results:
The eukaryotic expression vector pCI-CD34 was constructed, and stably transfected 3T3 cell line was established.
Conclusion:
Construction of eukaryotic expression vector of CD34 and the establishment of stably transfected 3T3 cell line are helpful to preparation of anti-CD34 mAbs and further functional study of CD34.
