Related Experiment Video
Updated: Jun 5, 2026
Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes
Published on: March 2, 2020
[Development of specific antibodies against ciprofloxacin]
Ping Li1, Sheng-liang Deng, Hong-bin Liu
1Institute of Quality Standard and Testing Technology for Agro-Product, Chinese Academy of Agriculture Sciences, Beijing 100081, China.
Aim:
To prepare specific antibodies against ciprofloxacin (CIP) and characterize the antibodies properities with ELISA, then lay the foundation for development of CIP rapid detection kit.
Methods:
New Zealand white rabbits and BALB/c mice were immunized with CIP-cBSA conjugate, CIP-nOVA and CIP-cOVA serving as coating antigen were used to screen antisera, indirect (competitive) ELISA was developed to determine the titer and specificity of the antisera.
Results:
Seven antisera against CIP were obtained, two of them were high specific to CIP with IC(50); 1 μg/L, the titer of which was 4 000 and 2 000, respectively.The antisera had some degree cross-reactivity with ENR, NOR and OFL, but had hardly cross-reactivity with PEN, KAN and QEN.
Conclusion:
The antibodies with high specificity developed in this study can be used to develop CIP detecting kit.
Related Concept Videos
01:28Inhibitors of Bacterial DNA Synthesis
01:30Development of Antibiotic Resistance