Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Excision Repair-Initiated Enzyme-Assisted Bicyclic Cascade Signal Amplification for Ultrasensitive Detection of Uracil-DNA Glycosylase.

Analytical chemistry·2017
Same author

Development of fluorescent methods for DNA methyltransferase assay.

Methods and applications in fluorescence·2017
Same author

Waist-hip Ratio (WHR), a Better Predictor for Prostate Cancer than Body Mass Index (BMI): Results from a Chinese Hospital-based Biopsy Cohort.

Scientific reports·2017
Same author

Chronic nicotine differentially affects murine transcriptome profiling in isolated cortical interneurons and pyramidal neurons.

BMC genomics·2017
Same author

Combinatorial Strategy to Identify Fluorescent Probes for Biothiol and Thiophenol Based on Diversified Pyrimidine Moieties and Their Biological Applications.

Analytical chemistry·2017
Same author

Highly Specific and Ultrasensitive Two-Photon Fluorescence Imaging of Native HOCl in Lysosomes and Tissues Based on Thiocarbamate Derivatives.

Analytical chemistry·2017

Related Experiment Video

Updated: Jun 5, 2026

Use of the Protease Fluorescent Detection Kit to Determine Protease Activity
09:00

Use of the Protease Fluorescent Detection Kit to Determine Protease Activity

Published on: August 4, 2009

A new specific fullerene-based fluorescent probe for trypsin.

Kehua Xu1, Fen Liu, Jing Ma

  • 1College of Chemistry, Chemical Engineering and Materials Science, Engineering Research Center of Pesticide and Medicine Intermediate Clean Production, Ministry of Education, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University, Jinan, 250014, PR China.

The Analyst
|January 7, 2011
PubMed
Summary
This summary is machine-generated.

A new fluorescent probe, C(60)-FL, enables direct and rapid detection of trypsin. This probe offers high selectivity and sensitivity, advancing enzyme detection methods.

More Related Videos

Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET
12:07

Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET

Published on: October 9, 2021

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
08:26

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

Related Experiment Videos

Last Updated: Jun 5, 2026

Use of the Protease Fluorescent Detection Kit to Determine Protease Activity
09:00

Use of the Protease Fluorescent Detection Kit to Determine Protease Activity

Published on: August 4, 2009

Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET
12:07

Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET

Published on: October 9, 2021

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
08:26

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Materials Science

Background:

  • Trypsin is a key enzyme in biological processes.
  • Developing selective and sensitive probes for enzyme detection is crucial.
  • Fluorescent probes offer advantages for real-time biological monitoring.

Purpose of the Study:

  • To design and synthesize a novel fluorescent probe for direct trypsin determination.
  • To investigate the mechanism of fluorescence change upon trypsin interaction.
  • To evaluate the probe's performance for trypsin quantification.

Main Methods:

  • Synthesis of a novel fluorescent probe (C(60)-FL) combining fluorescein and fullerene.
  • Utilizing photo-induced electron transfer (PET) mechanism for fluorescence detection.
  • Assessing probe response to varying trypsin concentrations and selectivity against other enzymes.

Main Results:

  • The C(60)-FL probe showed a fluorescence increase in the presence of trypsin due to inhibited PET.
  • Linear relationship observed between fluorescence intensity and trypsin concentration (4.40×10⁻⁷ to 7.04×10⁻⁵ g mL⁻¹).
  • Achieved a detection limit of 40 ng mL⁻¹ with high selectivity for trypsin over other enzymes.

Conclusions:

  • The C(60)-FL probe is effective for direct, rapid, and selective determination of trypsin.
  • The probe's properties facilitate its application in biological contexts for enzyme investigation.
  • This work contributes to the development of advanced fluorescent probes for biochemical analysis.