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Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos
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Embedding, serial sectioning and staining of zebrafish embryos using JB-4 resin

Jessica Sullivan-Brown1, Margaret E Bisher, Rebecca D Burdine

  • 1Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA.

Nature Protocols
|January 8, 2011
PubMed
Summary

This study presents a 3-day protocol for embedding zebrafish embryos in JB-4 plastic resin, enabling high-resolution visualization of cellular details. The method supports various staining techniques and signal preservation for advanced morphological studies.

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