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[Study on dynamic accumulation of index components from Bupleurum chinense in various collecting periods]
Qi-shuai Wang1, Xiao-kun Li, Yun Yang
1College of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China.
Objective:
To study the dynamic change law of volatile oil, saikosaponin a, d and alcohol-extract from Bupleurum chinense at Songxian region in Henan province, and to explore the optimal harvest period of Bupleurum chinense.
Methods:
With the contents of saikosaponin a and d, absorbance of volatile oil and percentage of alcohol-extract as indexes, HPLC-ELSD and ultraviolet spectrophotometry were successively used to analyze them.
Results:
There are obvious differences among the contents of volatile oil, saikosaponin a, d and alcohol-extract in various collecting periods sample, the absorption of volatile oil in distillation was the highest in October, the content of saikosaponin a was the highest in September, the saikosaponin d in December and the percentage of alcohol-extract in October.
Conclusion:
The optimal harvest period of Bupleurum chinense at Songxian region in Henan is identified, which can provide scientific basis for crude drug production and processing.