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Related Experiment Video

Updated: Jun 5, 2026

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
13:58

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays

Published on: September 26, 2011

Quantitative HIV-1 proviral DNA detection: a multicentre analysis.

Anita De Rossi1, Marisa Zanchetta, Francesca Vitone

  • 1Department of Oncology and Surgical Sciences, Oncology Section, Unit of Viral Oncology, AIDS Reference Centre, University of Padova, IOV-IRCCS, Padova, Italy.

The New Microbiologica
|January 11, 2011
PubMed
Summary

Standardizing human immunodeficiency virus type 1 (HIV-1) proviral DNA measurement is crucial. A multi-laboratory study achieved good agreement, paving the way for clinical application of proviral DNA load assessment.

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Area of Science:

  • Virology
  • Molecular Biology
  • Clinical Diagnostics

Background:

  • Standardized methods for measuring human immunodeficiency virus type 1 (HIV-1) proviral DNA are lacking, leading to discordant results across studies.
  • Accurate quantification of HIV-1 proviral DNA is essential for understanding viral load and clinical management.

Purpose of the Study:

  • To standardize a quantitative method for HIV-1 proviral DNA measurement across multiple laboratories.
  • To assess the DNA proviral load of the most relevant circulating HIV-1 subtypes.
  • To evaluate the inter-laboratory agreement for HIV-1 proviral DNA quantification.

Main Methods:

  • A study group of seven laboratories participated in a blind testing of reference samples.
  • Samples included cellular samples infected with HIV-1 clade B and peripheral blood mononuclear cells with varying concentrations of HIV-1 clade plasmids.
  • Quantification utilized the hTERT gene as a housekeeping gene and primers targeting the gag gene for different HIV-1 clades.

Main Results:

  • Inter-laboratory results showed no statistically significant differences, indicating good agreement among participating labs.
  • Minor variations were observed in HIV-1 DNA amounts and across different HIV clades.
  • The standardized method demonstrated reliability for quantifying HIV-1 proviral DNA.

Conclusions:

  • The developed method for HIV-1 proviral DNA quantification is standardized and shows good inter-laboratory agreement.
  • This standardization is a key step towards the clinical application of proviral DNA load assessment.
  • Further studies are needed to establish the clinical utility of proviral DNA load in managing HIV-1 infected patients.