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Related Concept Videos

Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...

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Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
08:50

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Published on: May 14, 2020

Epigenetics in alternative pre-mRNA splicing.

Reini F Luco1, Mariano Allo, Ignacio E Schor

  • 1National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.

Cell
|January 11, 2011
PubMed
Summary
This summary is machine-generated.

Alternative splicing, crucial for protein diversity, is increasingly understood to be regulated by epigenetic factors like chromatin structure. This suggests epigenetics influences not only gene expression but also RNA splicing outcomes.

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Area of Science:

  • Molecular Biology
  • Epigenetics
  • Genomics

Background:

  • Alternative splicing is a key mechanism generating protein diversity in eukaryotes.
  • Traditional research focused on RNA elements and splicing factors.
  • Emerging evidence highlights the role of chromatin structure in splicing regulation.

Purpose of the Study:

  • To explore the influence of chromatin structure and histone modifications on alternative splicing.
  • To integrate epigenetic regulation into the understanding of alternative splicing control.

Main Methods:

  • Analysis of RNA sequence elements and splicing factors.
  • Investigating the impact of chromatin modifications on splicing patterns.

Main Results:

  • Chromatin structure and histone modifications play a significant role in regulating alternative splicing.
  • Epigenetic mechanisms are integral to controlling how genes are spliced.

Conclusions:

  • Epigenetic regulation extends beyond gene expression to influence alternative splicing.
  • Understanding epigenetic control of splicing is vital for comprehending development and disease.