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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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Canine specific ELISA for coagulation factor VII.

Tom Knudsen1, Mads Kjelgaard-Hansen, Mikael Tranholm

  • 1Department of Small Animal Clinical Sciences, Faculty of Life Sciences, University of Copenhagen, Copenhagen DK-1870, Denmark. tmkn@novonordisk.com

Veterinary Journal (London, England : 1997)
|January 11, 2011
PubMed
Summary
This summary is machine-generated.

A new canine-specific assay for Factor VII antigen (FVII:Ag) was developed. This ELISA tool aids in diagnosing hereditary canine Factor VII deficiency, improving patient outcomes.

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Area of Science:

  • Veterinary Hematology
  • Canine Coagulation Disorders
  • Diagnostic Assay Development

Background:

  • Hereditary and acquired canine Factor VII (FVII) deficiency can lead to severe bleeding.
  • Current diagnostics primarily measure FVII activity (FVII:C), but canine-specific FVII antigen (FVII:Ag) assays are lacking.

Purpose of the Study:

  • To develop and validate a canine-specific enzyme-linked immunosorbent assay (ELISA) for measuring FVII:Ag in plasma.
  • To assess the utility of the FVII:Ag ELISA in diagnosing hereditary canine FVII deficiency.

Main Methods:

  • Development of a canine-specific ELISA for FVII:Ag quantification.
  • Validation of the ELISA using plasma samples from dogs with known FVII deficiency status.

Main Results:

  • The developed FVII:Ag ELISA demonstrated accuracy in diagnosing both homozygous and heterozygous hereditary FVII deficiency.
  • The assay provides a reliable method for measuring canine FVII:Ag levels.

Conclusions:

  • The canine-specific FVII:Ag ELISA is a valuable diagnostic tool for hereditary canine FVII deficiency.
  • Combined with FVII:C assays, this new ELISA enhances diagnostic capabilities for canine bleeding disorders.