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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Structure01:19

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...

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Related Experiment Video

Updated: Jun 5, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

RNA secondary structure in mutually exclusive splicing.

Yun Yang1, Leilei Zhan, Wenjing Zhang

  • 1Institute of Biochemistry, College of Life Sciences, Zhejiang University (Zijingang Campus), Hangzhou, Zhejiang, People's Republic of China.

Nature Structural & Molecular Biology
|January 11, 2011
PubMed
Summary
This summary is machine-generated.

This study reveals intronic RNA pairings that control mutually exclusive splicing, ensuring protein diversity. This mechanism, conserved across species, ensures precise exon selection for complex gene expression.

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Using the E1A Minigene Tool to Study mRNA Splicing Changes
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Last Updated: Jun 5, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
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Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Area of Science:

  • Molecular Biology
  • Genomics
  • RNA Biology

Background:

  • Mutually exclusive splicing is crucial for generating protein diversity but its mechanisms remain largely unknown.
  • Understanding these mechanisms is key to deciphering complex gene regulation and protein function.

Purpose of the Study:

  • To investigate the intronic elements controlling mutually exclusive splicing of 14-3-3ξ pre-mRNA.
  • To elucidate the conserved structural mechanisms underlying mutually exclusive splicing across different genes.

Main Methods:

  • Comparative genome analysis to identify conserved intronic elements.
  • RNA structure analysis to understand the functional role of these elements.
  • Investigating cis element interactions and RNA pairing competition.

Main Results:

  • Identified clade-specific yet evolutionarily conserved intronic elements in 14-3-3ξ pre-mRNA that control mutually exclusive splicing.
  • Demonstrated that inter-intronic RNA pairings have triple functions: activating proximal exons, repressing looped exons, and competing for RNA pairing.
  • Deciphered a similar structural code in Dscam and Mhc, suggesting a broadly applicable mechanism.

Conclusions:

  • A novel mechanism involving intronic RNA pairings ensures the selection of single exons during mutually exclusive splicing.
  • This mechanism is conserved at the secondary structure level and applicable to other complex gene systems like Dscam and Mhc.
  • Findings provide a fundamental insight into the regulation of protein diversity through alternative splicing.