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Related Concept Videos

Role of Microtubules in Cell Wall Deposition01:02

Role of Microtubules in Cell Wall Deposition

Microtubules are small hollow tubes in eukaryotic cells. The cell wall microtubules are polymerized dimers of two globular proteins, α-tubulin and β-tubulin, two globular proteins. With a diameter of about 25 nm, microtubules are the widest components of the cytoskeleton. They help the cell resist compression and provide a track along which vesicles move through the cell or pull replicated chromosomes to opposite ends of a dividing cell. Microtubules go through quick cycles of disassembly and...
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Every plant cell has a cell wall that protects the cell, provides structural support, and gives the cell shape. Cellulose, the main structural component of the plant cell wall, makes up over 30% of plant matter. It is the most abundant organic compound on earth.  Cellulose is an unbranched polysaccharide composed of linear chains of glucose molecules linked by β (1→4) glycosidic bonds.
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Updated: Jun 5, 2026

High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications
09:27

High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications

Published on: May 10, 2016

Analysing cellulose biosynthesis with confocal microscopy.

Meera Nair1, Seth Debolt

  • 1Department of Horticulture, University of Kentucky, Lexington, KY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 12, 2011
PubMed
Summary
This summary is machine-generated.

Plant cell walls enable growth and shape through controlled extension. This chapter details live cell imaging methods for cellulose synthase (CESA) in Arabidopsis, aiding the study of cell morphogenesis.

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Last Updated: Jun 5, 2026

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Label-free in situ Imaging of Lignification in Plant Cell Walls

Published on: November 1, 2010

Area of Science:

  • Plant Biology
  • Cell Biology
  • Biophysics

Background:

  • Plant cells possess a rigid cell wall that maintains turgor pressure while allowing controlled expansion for growth and morphogenesis.
  • Live cell fluorescence microscopy provides dynamic insights into protein behavior during cell shape development.
  • Cellulose synthase (CESA) is a key enzyme complex in plant cell wall biosynthesis.

Purpose of the Study:

  • To present methodologies for live cell imaging of cellulose synthase (CESA) in Arabidopsis.
  • To offer a framework for investigating the dynamic behavior of specific proteins involved in cell morphogenesis.
  • To enhance understanding of the molecular mechanisms underlying plant cell growth and shape determination.

Main Methods:

  • Utilizing live cell fluorescence microscopy techniques.
  • Focusing on the imaging of cellulose synthase (CESA) complexes in Arabidopsis thaliana.
  • Adapting established imaging protocols for visualization of specific protein dynamics.

Main Results:

  • Demonstration of effective live cell imaging protocols for CESA in Arabidopsis.
  • Visualization of CESA dynamics provides insights into cell wall construction during growth.
  • Methodology is adaptable for studying other proteins involved in cell morphogenesis.

Conclusions:

  • Live cell imaging of CESA in Arabidopsis is a powerful approach to study plant cell wall dynamics.
  • The presented methodologies facilitate the investigation of protein functions in cell morphogenesis.
  • This work provides a foundation for future research into the molecular mechanisms of plant cell growth and development.