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What is Genetic Engineering?00:49

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Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...

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Mouse Genome Engineering Using Designer Nucleases
12:04

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Published on: April 2, 2014

Genetic engineering: Processes and products.

J Davies1

  • 1Institut Pasteur, 28 rue du Dr Roux, 75724 Paris cedex 15, France.

Trends in Ecology & Evolution
|January 14, 2011
PubMed
Summary

Genetic engineering techniques are widely used in laboratories for pharmaceutical production. This review examines the capabilities and limitations of these methods for creating genetically engineered organisms.

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Pharmaceutical Science

Background:

  • Genetically engineered organisms are primarily confined to laboratory settings.
  • The fundamental techniques for genetic engineering are consistent across different applications.

Purpose of the Study:

  • To examine the fundamentals of genetic engineering in pharmaceutical production.
  • To illustrate the capabilities and limitations of current genetic engineering methods.

Main Methods:

  • Review of genetic engineering techniques.
  • Analysis of methods used in high-value pharmaceutical production.

Main Results:

  • Genetic engineering methods are largely consistent for laboratory strains and those intended for environmental release.

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  • Current methods demonstrate specific capabilities and limitations in pharmaceutical applications.

    • Conclusions:

    • The foundational genetic engineering techniques are applicable to both contained and released organisms.
    • Understanding the capabilities and limitations is crucial for advancing pharmaceutical biotechnology.