Related Experiment Video
Updated: Jun 5, 2026
Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation
Published on: April 3, 2017
Markers of adipose tissue macrophage content are negatively associated with serum HDL-C concentrations
Jianying He1, Xiaoyuan Xu, Ann Francisco
1Phoenix Epidemiology and Clinical Research Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Department of Health and Human Services, Phoenix, AZ 85016, USA. hejianying@mail.nih.gov
Objective:
Adipose tissue macrophages are associated with adiposity and may play a role in obesity related co-morbidities. We examined whether abdominal subcutaneous adipose tissue macrophage content (ATMc) was associated with fasting serum total cholesterol (TC), high density lipoprotein (HDL-C) and triglycerides (TG) concentrations.
Methods:
52 (35 male) of Pima Indians without diabetes participated in the study. ATMc was assessed by histochemistry (CD68+ cells) (Mphi) and gene expression of macrophage-specific markers including CD68, CSF1r, CD11b and CD11c.
Results:
HDL-C was negatively associated with CD68, CSF1R, CD11b and CD11c expression (r=-0.31, p=0.02; r=-0.38, p<0.01; r=-0.40, p<0.01; r=-0.26, p=0.04, respectively). After adjustment for age, sex and BMI, the associations between HDL-C and CSF1R, CD11b were still significant (r=-0.31, p=0.01 and r=-0.3, p=0.02), but borderline for CD68 (r=-0.24, p=0.06).
Conclusions:
ATMc is associated with lower HDL-C independent of BMI indicating a link between ATMc and cardiovascular risk factors.
More Related Videos
10:28Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
Published on: July 17, 2018
07:29Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
Related Concept Videos
01:29Cholesterol: Significance and Regulation
Considering cholesterol and...
01:25Blood Studies for Cardiovascular System III: Serum Lipid Profile
Serum lipids are fats and fatty substances in the blood and are crucial for various bodily functions, including energy storage, cellular structure, and hormone production. Serum lipids consist of cholesterol, triglycerides, and phospholipids.
Cholesterol is a soft, fat-like substance found in all body cells. It is crucial for producing hormones, vitamin D, and substances that aid...
01:38Inflammation