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Characterization of an Aspergillus oryzae cysteinyl dipeptidase expressed in Escherichia coli
Ryota Hattori1, Mayumi Matsushita-Morita, Junichiro Marui
1National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
Bioscience, Biotechnology, and Biochemistry
|January 14, 2011
Abstract:
Cysteinyl dipeptidase from Aspergillus oryzae (CdpA) was produced in Escherichia coli and purified. The enzyme showed activity specific toward cysteine-containing dipeptides, but its substrate specificity was distinct from those of other cysteinyl dipeptidases of the M20 family. It was optimally active at pH 7-8 and stable at pH 6-9 and at up to 40 °C.
