Related Experiment Video
Updated: Jun 5, 2026

Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG
Published on: May 16, 2017
Molecular cloning and sequence analysis of two distinct halotolerant extracellular proteases from Bacillus subtilis
Shinji Takenaka1, Nobutaka Yoshida, Ken-ichi Yoshida
1Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe 657-8501, Japan. hakko3@kobe-u.ac.jp
Abstract:
We cloned the genes encoding the two distinct extracellular halotolerant proteases of Bacillus subtilis FP-133 Expro-I and Expro-II, which were classified as alkaline serine and neutral proteases respectively. Three-dimensional modeling suggested that acidic and polar amino acid residues located on the surface stabilize protein structure in the presence of relatively high NaCl concentrations.
Related Concept Videos
Modern Molecular Taxonomy
Applications of Molecular Taxonomy

