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Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast
Published on: January 23, 2012
Fluorescence energy transfer efficiency in labeled yeast cytochrome c: a rapid screen for ion biocompatibility in
Sheila N Baker1, Hua Zhao, Siddharth Pandey
1Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37931, USA.
Physical Chemistry Chemical Physics : PCCP
|January 14, 2011
Abstract:
A fluorescence energy transfer "de-quenching" assay was implemented to follow the equilibrium unfolding behaviour of site-specific tetramethylrhodamine-labelled yeast cytochrome c in aqueous ionic liquid solutions; additionally, this approach offers the prospect of naked eye screening for biocompatible ion combinations in hydrated ionic liquids.
