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Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

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Related Experiment Video

Updated: Jun 5, 2026

Optical Frequency Domain Imaging of Ex vivo Pulmonary Resection Specimens: Obtaining One to One Image to Histopathology Correlation
14:21

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Path-length-resolved diffusive particle dynamics in spectral-domain optical coherence tomography.

J Kalkman1, R Sprik, T G van Leeuwen

  • 1Biomedical Engineering & Physics, Academic Medical Center, University of Amsterdam, P.O. Box 22700, 1100 DE Amsterdam, The Netherlands. j.kalkman@amc.nl

Physical Review Letters
|January 15, 2011
PubMed
Summary

We developed a new Fourier-domain optical coherence tomography (FD-OCT) method to measure particle diffusion dynamics. This technique enhances imaging speed by 200x, enabling detailed analysis of complex samples.

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Last Updated: Jun 5, 2026

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Area of Science:

  • Biomedical Optics
  • Optical Coherence Tomography
  • Diffusive Dynamics Imaging

Background:

  • Optical Coherence Tomography (OCT) is crucial for non-invasive imaging.
  • Measuring diffusive particle dynamics in concentrated samples is challenging.
  • Existing methods lack sufficient spatial and temporal resolution.

Purpose of the Study:

  • To introduce a novel method for measuring the decorrelation rate of OCT magnitude.
  • To enhance particle diffusion imaging speed and resolution.
  • To enable quantitative studies of diffusive dynamics in complex, high-concentration samples.

Main Methods:

  • Utilized Fourier-domain OCT (FD-OCT) for enhanced sensitivity.
  • Varied sphere diameter to cover a range of translational diffusion coefficients.
  • Employed coherent gating to minimize multiple scattering effects.

Main Results:

  • Achieved a 200-fold increase in particle diffusion imaging speed compared to time-domain OCT.
  • Successfully measured diffusive particle dynamics in high-concentration samples.
  • Demonstrated simultaneous imaging of morphology and diffusive dynamics with high spatial and temporal resolution.

Conclusions:

  • The new FD-OCT method significantly advances the study of diffusive particle dynamics.
  • This technique is suitable for analyzing complex biological tissues and other concentrated samples.
  • Offers a powerful tool for simultaneous morphological and dynamic characterization.