Reliability of EMG determinism to detect changes in motor unit synchrony and coherence during submaximal contraction

The determinism (DET) is a parameter used in nonlinear analysis to quantify the occurrence of recurrent patterns in a signal. Applied to the electromyographic activity (EMG), DET has been proposed as an index of motor unit synchrony in human. We have recently shown that the amount of motor unit synchronous firings above chance level was enhanced with stronger submaximal muscle contraction. Using these data, we aimed at determining if (1) EMG DET and motor unit synchrony varied in the same way and (2) EMG DET was more specifically related to the degree of oscillatory coupling between motor unit discharges. Cross-correlation and coherence analyses were applied to the discharges of 30 motor unit pairs tested at various force levels to assess the amount of synchronous impulses and the strength of oscillatory coupling in the time and frequency domains, respectively. Recurrent quantification analysis was applied to EMG activity to extract its DET. Overall, changes in EMG DET were poorly explained by changes in motor unit synchronous impulse probability (6%) and frequency (5%), and by changes in motor unit coherence in the 6-12Hz (5%) and 25-40Hz (8%) bands. Moreover, the comparison of the data obtained at the weakest and the strongest contraction levels tested with each motor unit pair showed that EMG DET remained unaltered with stronger contraction despite the occurrence of consistent changes in motor unit synchrony in both time and frequency domains. This speaks strongly against the reliability of DET in evaluating changes in motor unit synchronization during submaximal muscle contraction.