Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Discordance between measured and calculated total carbon dioxide.

J P Ungerer1, M J Ungerer, W J Vermaak

  • 1Department of Chemical Pathology, Faculty of Medicine, University of Pretoria, South Africa.

Clinical Chemistry
|December 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Validation of NICE diagnostic guidance for rule out of myocardial infarction using high-sensitivity troponin tests.

Heart (British Cardiac Society)·2016
Same author

Biological variation of ischaemia-modified albumin in healthy subjects.

Cardiovascular journal of Africa·2008
Same author

Gas chromatographic-mass spectrometric method for quantitation of phenylalanine and tyrosine in serum.

Journal of chromatography. B, Biomedical sciences and applications·2001
Same author

Significance of cerebrospinal fluid adenosine deaminase isoenzymes in tuberculous (TB) meningitis.

Journal of clinical laboratory analysis·2001
Same author

Novel test and its automation for the determination of erythrocyte acetylcholinesterase and its application to organophosphate exposure.

Clinica chimica acta; international journal of clinical chemistry·2001
Same author

Effect of magnesium supplementation on the fractional intestinal absorption of 45CaCl2 in women with a low erythrocyte magnesium concentration.

Metabolism: clinical and experimental·2000

Accurate measurement of total carbon dioxide (TCO2) is crucial. This study demonstrates that strict pre-analytical control ensures excellent agreement between measured and calculated TCO2, supporting reliable clinical acid-base status evaluation.

Area of Science:

  • Clinical Chemistry
  • Biochemistry
  • Acid-Base Balance

Background:

  • Conflicting results exist regarding the agreement and correlation between measured and calculated total carbon dioxide (TCO2).
  • Pre-analytical variations are suspected contributors to these discrepancies in TCO2 measurements.

Purpose of the Study:

  • To investigate the correlation and agreement between measured and calculated TCO2 under minimized pre-analytical variation.
  • To assess the impact of sample handling on TCO2 measurement accuracy.
  • To determine if either measurement method can be reliably used for clinical acid-base status evaluation.

Main Methods:

  • Analyzed 88 samples with stringent control of pre-analytical variables.
  • Evaluated 913 routine clinical samples.

Related Experiment Videos

  • Assessed correlation (r) and agreement (standard deviation of differences) between measured and calculated TCO2.

    • Main Results:

    • Excellent correlation (r = 0.98) and small agreement variation (SD = 1.1 mmol/L) were observed in the controlled sample set.
    • Similar results were obtained in a larger set of routine samples.
    • Discrepancies in literature may stem from variations in sample types and handling procedures.

    Conclusions:

    • Rigid control of pre-analytical procedures is essential for accurate TCO2 measurement studies.
    • Both measured and calculated TCO2 methods demonstrate agreement over a wide range.
    • Either method can be reliably employed for accurate clinical acid-base status assessment.